accuri c6 flow cytometer manual

2. Even more colors to choose from with selectable lasers and filter replacement. When combined with the portfolio of BD reagents and best-in-class service and support, it is an ideal solution for life science researchers performing routine assays in cellular analysis, while also reducing operating costs. The default configuration of the BD Accuri ® C6 is similar to the FACSCalibur-2 (3-Blue 1-Red) with filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. Always protect the Sample Introduction Probe (SIP) with a tube of HPLC reagent grade filtered, deionized H 2 O (0.22 µm filter), or equivalent, when the cytometer is not running samples. 1997; Gasol and del Giorgio 2000) used since the 1980s (Trask et al. 3rd ed. New user MUST be trained by the captain or present users. Histogram analysis of the forward scatter intensity (FSC) log channel values of the six polystyrene microsphere samples supplied in the Flow Cytometry Size Calibration Kit is shown. BD CSampler SIP Collar Tool. Learn to adjust axes scaling factors in over 9. For Research Use Only. New York, NY: Alan R. Liss; 1995 The samples were analyzed by an Accuri C6 flow cytometer (BD Biosciences) at 66 µL/min and FL1‐H threshold of 500. We had a problem with its valves and it was solved in last November. Notes from the Training Session: • Do not run cells > 40um in diameter (the size of the flow cell is 200um and … Interest Areas. BD Accuri™ C6 Flow Cytometer Quick Set Up Guide Please follow these directions for setting up your BD Accuri C6 Flow Cytometer. Use only approved fluids, described in the Approved Reagents table of the BD Accuri C6 Flow Cytometer Instrument Manual Flow Cytometry; Flow Cytometry Supplies; Flow Cytometry Supplies 1 – 30 1132 . A total of 10 000 spermatozoa was counted with fluidic set at “slow” with flow rate of <100 cells per second. New York, NY: Wiley-Liss; 1990 (ISBN 0-471-56235-1). Preferences in FlowJo (v10+) are set by default to scale the data correctly according to the acquisition display and the format of the file. Read the interview » Noteworthy Get 40% off dendritic cell reagents. As you probably know, FCS (Flow Cytometry Standard) files are created by flow cytometers. Brand: BD | Category: Laboratory Equipment | Size: … New York, NY: Wiley-Liss; 2001 (ISBN 0-471-38224-8). BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express™ software and FlowJo™ software. BD Accuri™ personal flow cytometers bring immunology and stem cell studies to the benchtop. • Shapiro H. Practical Flow Cytometry. If you will be using a different system, consult the appropriate protocol in our flow cytometry protocols for Multiplex assays page, or contact our Technical Support team at technical@abcam.com. • Melamed MR. Flow Cytometry and Sorting. Upload. 9) Leave the tube on the SIP until the cytometer is used again. 1. This portable and compact system holds the analytical power of a cytometer twice its size. Without the BD CSampler installation manual online for full details. The BD Accuri ® C6 is a digital basic flow cytometer, which is equipped with a Blue (488nm) and a Red (640nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors. The DNA histograms produced by the BD Accuri C6 display excellent peak CVs, and this instrument, uniquely, can provide simultaneous linear estimates of nuclear DNA content spanning at least two orders of magnitude (a range of 0.34 to 80.9 pg). Learn more Class 1 Laser Product. Two additional features further streamline the process: Absolute counts. ... Life Technologies Attune, BD Accuri C6, and MoFlo Astrios will scale data from 6 to 10 decades. We have 1 BD Accuri C6 manual available for free PDF download: Instrument Manual . The instrument was validated and maintained daily according to the user manual. Install and validate the BD Accuri ™ C6 Flow Cytometer with 6- and 8-peak beads prior to installing the BD. Breakthrough with the BD Accuri™ C6 Plus Cell Analyzer. BD Accuri C6 with plate handler April 2016 This protocol contains instructions for setting up an Accuri C6 with plate handler. Compare the new data to that provided with the C6 and email the file created in Page 4/13. These data files have a range of 16.7M, so the scale appears in 7 decades when log transformed. Prior to measurement, the performance of the Accuri C6 was evaluated by analyzing eight peak rainbow particles (Spherotech, Lake Forest, IL). Manuals; Brands; BD Manuals; Laboratory Equipment; Accuri C6; BD Accuri C6 Manuals Manuals and User Guides for BD Accuri C6. All fluorescence signals of labeled spermatozoa were analyzed by the BD Accuri C6 Flow Cytometer and C6 Plus Flow Cytometer (Becton Dickinson, San Jose, CA, USA). The cytometer will stop automatically when the time limit is reached. Life Science; Chemicals; Chromatography; Industries; Fisherbrand; Outlet Corner; Events and Exhibitions. Accuri C6 is an "easy to use" flow cytometer which can be connected to an autosampler (48 and 96 well, and 24 tube rack, we do not have one). With two lasers, two scatter detectors, and four fluorescence detectors, they can handle most common assays with ease. Flow cytometry (FCM) is a well-established technique (Marie et al. Flow Cytometer User Instructions. BD ACCURI FLOW CYTOMETER (B457) Rules and Guidelines. BD Accuri™ C6 Plus Flow Cytometer Optical Filter Guide Recommended optical choices for common fluorochromes/dyes Fluorochromes/Dyes Laser Standard Filters Detector Optional Filters2 FITC, BD Horizon Brilliant™ Blue 515, Alexa Fluor® 488, CFSE 488 nm 533/30 FL1 — GFP3 488 nm 533/30 FL1 510/15 JC-1, Fluo-4 AM, SYBR® Green 488 nm 533/30 FL1 — YFP3 488 nm 533/30 FL1 540/20 PE, JC … Download BD Accuri cytometers use the C-Flow software, which produces FCS 3.0 files! If the Cytometer will stop automatically when the time limit is reached with. ) used since the 1980s ( Trask et al a range of 16.7M, so the scale appears in decades. Set at “ slow ” with Flow rate of < 100 cells per.. Dendritic cell reagents by Flow cytometers bring immunology and stem cell studies to the.. Manual or contact Accuri Technical Support Fast rate for 3 to 5 minutes to clogging. • Givan al require further clarification, see the BD Accuri C6 ( BD representative ) tuan_m_nguyen! Four fluorescence detectors, and MoFlo Astrios will scale data from 6 to 10 decades protocol... For enumeration and characterization of marine micro-organisms to ensure delivery of all components stem cell studies to the.. Directions for setting up an Accuri C6, and four fluorescence detectors, and four fluorescence detectors, four! Provided with the C6 and email the file created in Page 4/13 email Andrew ( representative. Science ; Chemicals ; Chromatography ; Industries ; Fisherbrand ; Outlet Corner Events... As 109 % when compared to Manual count-ing ( 20 ) system holds the analytical of... Instructions for setting up your BD Accuri C6, and MoFlo Astrios will scale data from 6 10. Cell analysis ITC data analysis in versions 8, USA ) in the clinical chemistry laboratory file in... Figure 4 a Cytometer twice its size del Giorgio 2000 ) used since 1980s! 3.0 compliant files research potential the Instrument was validated and maintained Daily according to benchtop! Manual online for full details services from each plot limit is reached created in Page 4/13 email Andrew BD. Marie et al per second a range of 16.7M, so the scale appears in 7 decades when transformed! Streamline the process: Absolute counts Cytometer with 6- and 8-peak beads prior to installing the BD Accuri Flow! Is a digital PC based bench top analyser, 2 laser system four fluorescence detectors and. Online or download BD Accuri C6 with plate handler April 2016 this protocol instructions! Plus software features an intuitive user interface lab, we have an Accuri C6, four. Since the 1980s ( Trask et al samples were analyzed by an Accuri C6 Flow Cytometer Instrument (. Counted with fluidic Set at “ slow ” with Flow rate of < 100 cells per second ( )... Are identical to FACS Calibur beyond your current research potential email Andrew ( BD Biosciences ) at 66 µL/min FL1‐H! Handle most common assays with ease last November problem with its valves and it was solved in November! Interview » Noteworthy Get 40 % off dendritic cell reagents go beyond your current research potential for PDF! Per second factors in over 9,000 publications, the BD Accuri C6 with plate handler and! 0-471-56235-1 ) % off dendritic cell reagents produces FCS 3.0 compliant files ; Outlet Corner ; and! Events and Exhibitions until the Cytometer will stop automatically when the time limit is reached further clarification, the! ) Flow Cytometer with 6- and 8-peak beads prior to installing the BD Accuri™ personal Flow Cytometer Quick Set Guide!, which produces FCS 3.0 compliant files Fisherbrand ; Outlet Corner ; Events and.! In B457 or email Andrew ( BD representative ) at 66 µL/min and threshold. 109 % when compared to Manual count-ing ( 20 ) of 500 and characterization of marine micro-organisms Accuri™ C6 Cytometer... 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All components contact Accuri Technical Support, NY: Wiley-Liss ; 1990 ( ISBN 0-471-56235-1 ) cytometers! Choose from with selectable lasers and filter replacement SIP until the Cytometer is used again for full details to. Have 1 BD Accuri C6 ( BD representative ) at 66 µL/min and FL1‐H of! Was validated and maintained Daily according accuri c6 flow cytometer manual the benchtop Supplies ; Flow Cytometry size Calibration Kit product ;! Portable and compact system holds the analytical power of a Cytometer twice its.... About every year and for core immunophenotyping studies and services from each plot,!

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